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zo 1  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology zo 1
    The effects of naringin and osthole alone or in combination on DSS-induced intestinal barrier. A Immunofluorescence staining analysis <t>of</t> <t>ZO-1</t> (green) and Occludin (red) in colon tissue. Representative images are displayed. Scale bar = 100 µm. B RT‒qPCR for IL-6, IL-1β, TNF-α and IL-10 in colon sections. C Detection of myeloperoxidase activity (MPO). D – I Western blotting and quantitative analysis of apoptosis factors Bcl-2, Bax, and Cleaved-Caspase-3. Data are presented as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001
    Zo 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zo+2/6%2C6-Dimethyl-1%2C2%2C3%2C6-tetrahydro-pyrazine/pmc12868363-20-0-10
    Average 94 stars, based on 1 article reviews
    zo 1 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Combined treatment with naringin and osthole ameliorates colitis through microbiota–amino acid metabolism and the JNK pathway"

    Article Title: Combined treatment with naringin and osthole ameliorates colitis through microbiota–amino acid metabolism and the JNK pathway

    Journal: Natural Products and Bioprospecting

    doi: 10.1007/s13659-025-00582-z

    The effects of naringin and osthole alone or in combination on DSS-induced intestinal barrier. A Immunofluorescence staining analysis of ZO-1 (green) and Occludin (red) in colon tissue. Representative images are displayed. Scale bar = 100 µm. B RT‒qPCR for IL-6, IL-1β, TNF-α and IL-10 in colon sections. C Detection of myeloperoxidase activity (MPO). D – I Western blotting and quantitative analysis of apoptosis factors Bcl-2, Bax, and Cleaved-Caspase-3. Data are presented as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001
    Figure Legend Snippet: The effects of naringin and osthole alone or in combination on DSS-induced intestinal barrier. A Immunofluorescence staining analysis of ZO-1 (green) and Occludin (red) in colon tissue. Representative images are displayed. Scale bar = 100 µm. B RT‒qPCR for IL-6, IL-1β, TNF-α and IL-10 in colon sections. C Detection of myeloperoxidase activity (MPO). D – I Western blotting and quantitative analysis of apoptosis factors Bcl-2, Bax, and Cleaved-Caspase-3. Data are presented as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

    Techniques Used: Immunofluorescence, Staining, Activity Assay, Western Blot

    Related Articles

    Blocking Assay:

    Article Title: Neuroprotective effects of salvianolic acids combined with Panax notoginseng saponins in cerebral ischemia/reperfusion rats concerning the neurovascular unit and trophic coupling
    Article Snippet: .. Primary antibodies anti‐ZO‐1 at 1:1000 (sc‐33725; Santa Cruz Biotechnology,), anti‐ZO‐2 at 1:1000 (sc‐515115; Santa Cruz Biotechnology), and anti‐JAM‐1 at 1:1000 (sc‐53623; Santa Cruz B) at 4°C overnight after blocking. ..

    Incubation:

    Article Title: Effect of short-term gravitational changes on the human minor salivary gland stem cell characteristics.
    Article Snippet: Objectives: Human minor salivary gland stem cells (huMSGSCs) are promising in regenerative medicine.. Their multipotent capabilities enable tissue regeneration and offer treatment potential for various diseases.. The effects of hypergravity (HyperG) and microgravity (MicroG) on stemness and therapeutic potential are not well explored.

    Western Blot:

    Article Title: c‐Abl Kinase Targets Tight Junction Protein ZO ‐2 in Regulation of Cell Migration and Morphology
    Article Snippet: .. Antibodies used in Western blot analysis were c‐Abl monoclonal antibody (K2, Santa Cruz), flag monoclonal antibody (Sigma‐Aldrich, Cat. no. F3165, RRID: AB_259529), ZO‐2 polyclonal antibody (Santa Cruz, sc‐11448 [H‐110], RRID: AB_2203583), phospho‐tyrosine monoclonal antibody (4G10, Cell Signaling Technology, Cat. no. 96215, RRID: AB_3096295), β‐actin monoclonal antibody (Sigma‐Aldrich, Cat. no. A5441, RRID: AB_476744), Jak1 polyclonal antibody (Cell Signaling Technology, Cat. no. 3332, RRID: AB_2128499), and phospho‐Jak1 (Tyr1022/1023) polyclonal antibody (Cell Signaling Technology, Cat. no. 3331, RRID: AB_2265057). ..

    Membrane:

    Article Title: The manganese transporter SLC39A8 links alkaline ceramidase 1 to inflammatory bowel disease
    Article Snippet: Protein samples were loaded on SDS-polyacrylamide gels, separated by electrophoresis, and then transferred to a nitrocellulose membrane (Bio-Rad, Hercules, CA, USA; Cat. No. 1620115). .. The membrane was immunoblotted with primary antibody ZO-1 (Santa Cruz, sc-33725, 1:500), ZO-2 (Santa Cruz; sc-515115, 1:500), Claudin-2 (Thermo Fisher, # 51-6100, 1:1000), Claudin-3 (Thermo Fisher, # 34-1700, 1:1000), Claudin-5 (Thermo Fisher, # 35-2500, 1:1000), Claudin-7 (Thermo Fisher, # 34-9100, 1:1000), Occludin (Santa Cruz, sc-133256, 1:1000), Cytokeratin 8/18 (Abcam, ab53280, 1:50000), ASAH3 antibody (Thermo Fisher, # PA5-75603, 1:500), and Actin (Proteintech, 66009-1-lg, 1:3000). .. The blots were visualized with infrared secondary antibodies, IRDye 680RD Goat anti-rat IgG Secondary antibody (Liborbio, #926-69076, 1:20000), IRDye 800CW Donkey anti-mouse IgG Secondary antibody (Liborbio, #925-32212, 1:20000), or IRDye 680LT Donkey anti-rabbit IgG Secondary antibody (Liborbio, #926-69021, 1:15000), using a LI-COR Odyssey fluorescent western blotting system (LI-COR Biosciences, Lincoln, NE, USA).



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    zo 1  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology zo 1
    The effects of naringin and osthole alone or in combination on DSS-induced intestinal barrier. A Immunofluorescence staining analysis <t>of</t> <t>ZO-1</t> (green) and Occludin (red) in colon tissue. Representative images are displayed. Scale bar = 100 µm. B RT‒qPCR for IL-6, IL-1β, TNF-α and IL-10 in colon sections. C Detection of myeloperoxidase activity (MPO). D – I Western blotting and quantitative analysis of apoptosis factors Bcl-2, Bax, and Cleaved-Caspase-3. Data are presented as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001
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    HYP ameliorates intestinal inflammation and restores barrier integrity in DSS-induced colitis mice. mRNA expression levels of (A) IL-6, (B) IL-1β, and (C) TNF-α in colon tissues. Levels of (E) IL-6, (F) IL-1β, and (G) TNF-α in serum. Quantitative analysis of the positive area percentage for <t>(D)</t> <t>Mucin-2</t> and (H) ZO-1. Representative images of (I) Mucin-2 immunofluorescence and (J) ZO-1 immunohistochemistry staining in colon tissues. Data are expressed as means ± SD (n=4-6). Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test for multiple comparisons. **P < 0.01, *** P < 0.001, and **** P < 0.0001.
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    HYP ameliorates intestinal inflammation and restores barrier integrity in DSS-induced colitis mice. mRNA expression levels of (A) IL-6, (B) IL-1β, and (C) TNF-α in colon tissues. Levels of (E) IL-6, (F) IL-1β, and (G) TNF-α in serum. Quantitative analysis of the positive area percentage for <t>(D)</t> <t>Mucin-2</t> and (H) ZO-1. Representative images of (I) Mucin-2 immunofluorescence and (J) ZO-1 immunohistochemistry staining in colon tissues. Data are expressed as means ± SD (n=4-6). Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test for multiple comparisons. **P < 0.01, *** P < 0.001, and **** P < 0.0001.
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    Image Search Results


    The effects of naringin and osthole alone or in combination on DSS-induced intestinal barrier. A Immunofluorescence staining analysis of ZO-1 (green) and Occludin (red) in colon tissue. Representative images are displayed. Scale bar = 100 µm. B RT‒qPCR for IL-6, IL-1β, TNF-α and IL-10 in colon sections. C Detection of myeloperoxidase activity (MPO). D – I Western blotting and quantitative analysis of apoptosis factors Bcl-2, Bax, and Cleaved-Caspase-3. Data are presented as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

    Journal: Natural Products and Bioprospecting

    Article Title: Combined treatment with naringin and osthole ameliorates colitis through microbiota–amino acid metabolism and the JNK pathway

    doi: 10.1007/s13659-025-00582-z

    Figure Lengend Snippet: The effects of naringin and osthole alone or in combination on DSS-induced intestinal barrier. A Immunofluorescence staining analysis of ZO-1 (green) and Occludin (red) in colon tissue. Representative images are displayed. Scale bar = 100 µm. B RT‒qPCR for IL-6, IL-1β, TNF-α and IL-10 in colon sections. C Detection of myeloperoxidase activity (MPO). D – I Western blotting and quantitative analysis of apoptosis factors Bcl-2, Bax, and Cleaved-Caspase-3. Data are presented as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

    Article Snippet: ZO-1 , sc-33725 , 1: 200 (IF) , Mouse , Santa Cruz Biotechnology.

    Techniques: Immunofluorescence, Staining, Activity Assay, Western Blot

    HYP ameliorates intestinal inflammation and restores barrier integrity in DSS-induced colitis mice. mRNA expression levels of (A) IL-6, (B) IL-1β, and (C) TNF-α in colon tissues. Levels of (E) IL-6, (F) IL-1β, and (G) TNF-α in serum. Quantitative analysis of the positive area percentage for (D) Mucin-2 and (H) ZO-1. Representative images of (I) Mucin-2 immunofluorescence and (J) ZO-1 immunohistochemistry staining in colon tissues. Data are expressed as means ± SD (n=4-6). Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test for multiple comparisons. **P < 0.01, *** P < 0.001, and **** P < 0.0001.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Microbiota-driven therapeutic efficacy of Hyperoside in ulcerative colitis and associated anxiety

    doi: 10.3389/fcimb.2026.1734356

    Figure Lengend Snippet: HYP ameliorates intestinal inflammation and restores barrier integrity in DSS-induced colitis mice. mRNA expression levels of (A) IL-6, (B) IL-1β, and (C) TNF-α in colon tissues. Levels of (E) IL-6, (F) IL-1β, and (G) TNF-α in serum. Quantitative analysis of the positive area percentage for (D) Mucin-2 and (H) ZO-1. Representative images of (I) Mucin-2 immunofluorescence and (J) ZO-1 immunohistochemistry staining in colon tissues. Data are expressed as means ± SD (n=4-6). Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test for multiple comparisons. **P < 0.01, *** P < 0.001, and **** P < 0.0001.

    Article Snippet: The following antibodies were used in this study: anti-ZO-1 antibody (AF5145, Affinity), anti-mucin-2 antibody (GB11344, Serbicebio), anti-BDNF antibody (GB11559, Serbicebio), anti-GFAP antibody (GB11096, Serbicebio), anti-Iba-1 antibody ( GB154490 , Serbicebio), NF-κB p65 (Proteintech, 10745-1-AP), Phospho-NF-κB p65 (Affinity, AF2006), AKT (Proteintech, 10176-2-AP), Phospho-AKT (Proteintech, 28731-1-AP), p38MAPK (Proteintech, 51115-1-AP), Phospho-p38MAPK (Affinity, AF4001).

    Techniques: Expressing, Immunofluorescence, Immunohistochemistry, Staining